This is the original paper on the method Choi optimized. It's pretty dense. Also read the Choi paper I just posted to get a better idea of potential applications.

Harry Choi, a grad student in Niles Pierce's lab (Caltech) has developed a new type of mRNA in situ hybridization. Could this become the mRNA-FISH that works in prokaryotes???

"Although syntrophic coupling between hydrogen producers and consumers is a major feature of the carbon cycle, mechanisms for energy recovery at the extremely low free energies of reactions typical of these anaerobic communities have not been established. In this study, comparative transcriptional analysis of a model sulfate-reducing microbe, Desulfovibrio vulgaris Hildenborough, suggested the use of alternative electron transfer systems dependent on growth modality."