A few weeks ago, Jason Kelly explained in his post how Itaya and colleagues (2007) assembled the complete 135 kb rice chloroplast circular genome starting from a collection of 5-6 kb fragments and using sequential in vivo homologous recombination in Bacil

Postgenomic

A few weeks ago, Jason Kelly explained in his post how Itaya and colleagues (2007) assembled the complete 135 kb rice chloroplast circular genome starting from a collection of 5-6 kb fragments and using sequential in vivo homologous recombination in Bacillus

http://blog-msb.embo.org/blog/2008/01/jcvi10.html

Genome transplantation in bacteria: changing one species to another. Lartigue C (2007) Science.

Highly selective isolation of human DNAs from rodent-human hybrid cells as circular yeast artificial chromosomes by transformation-associated recombination cloning. Larionov V. (1996) Proceedings of the National Academy of Sciences.

Complete chemical synthesis, assembly, and cloning of a Mycoplasma genitalium genome. Gibson DG (2008) Science.

Bottom-up genome assembly using the Bacillus subtilis genome vector. Itaya M (2008) Nat Methods.

January 26, 2008