|
JournalFire makes it easy to share and discuss journal articles. Learn more...
|
Genome transplantation in bacteria: changing one species to another.
Science. 2007 Aug 3; 317(5838): 632-8.
Abstract
As a step toward propagation of synthetic genomes, we completely replaced the genome of a bacterial cell with one from another species by transplanting a whole genome as naked DNA. Intact genomic DNA from Mycoplasma mycoides large colony (LC), virtually free of protein, was transplanted into Mycoplasma capricolum cells by polyethylene glycol-mediated transformation. Cells selected for tetracycline resistance, carried by the M. mycoides LC chromosome, contain the complete donor genome and are free of detectable recipient genomic sequences. These cells that result from genome transplantation are phenotypically identical to the M. mycoides LC donor strain as judged by several criteria.
Article Feed
Anne Dekas (Caltech) is following 5 new articles in Caltech Ge/Bi 246: Environmental Viruses: Seminar Additional ReadingLogin or register to post comments
Login or register to post commentsA few weeks ago, Jason Kelly explained in his post how Itaya and colleagues (2007) assembled the complete 135 kb rice chloroplast circular genome starting from a collection of 5-6 kb fragments and using sequential in vivo homologous recombination in Bacillus
http://blog-msb.embo.org/blog/2008/01/jcvi10.html
- Login or register to post comments
A while back I wrote about Synthia and now a recent article published in Science shows some more of the remarkable work being done at the Craig Venter Institute with bacteria and their genetics.The research I’m referring seems to take a good step forward
http://my.biotechlife.net/2007/08/21/sr-anderson-mi-genome-es-su-genome/
- Login or register to post comments
For the past few days I've been rushing around, first to Woods Hole, Massachusetts, to talk to some people at the Marine Biological Laboratory about the E. coli book, and then on an infinite chain of connecting flights to come out to Aspen to participate in
http://scienceblogs.com/loom/2007/06/30/hither_and_yon.php
- Login or register to post comments
The folks at the Craig Venter Institute, having patented the technology for creating a synthetic organism, now have at least part of the process working: they report that they can take an entire bacterial genome from one organism and pop it into another, essentially
http://www.gnxp.com/blog/2007/06/genome-transplantation-in-bacteria.php
- Login or register to post comments
Two science news articles (here and here) about J. Craig Venter's efforts to hack the genome (or perhaps, more broadly, hacking microbiology) reminded me of a few other articles about his goals. The two articles I ran across today concern a rather cool experiment
http://cs.unm.edu/~aaron/blog/archives/2007/06/hacking_microbi.htm
- Login or register to post comments
The guy who raced the government to a draw sequencing the human genome took a step toward synthesizing life yesterday, when he and his colleagues reported transforming one organism into another. J. Craig Venter and scientists at the institute he runs were able
http://feeds.wsjonline.com/~r/wsj/health/feed/~3/128963263/